COMPARATIVE EFFECTS OF CONTINUOUS RADIO FREQUENCY CURRENT AT 50°C AND PULSED RF ON CYTOTOXICITY, MICROVESICLE SHEDDING, AND MRNA EXPRESSION FOR POMC IN HUMAN MONOCYTIC CELLS
ESRA Academy. Azma T. Sep 9, 2016; 138373; 0213 Topic: MISCELLANEOUS
Dr. Toshiharu Azma
Dr. Toshiharu Azma

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Abstract
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Background and Aims:

Modality and settings for temperature depend on the specification of radio frequency (RF) generators.  A newly developed RF generator, TLG-10 (TOP, Tokyo), is equipped to generate continuous RF at 50°C.  Effects of continuous RF at such relatively low temperature on cellular viability remain unknown.  Because parenchymal glial cells is suggested to be recruited from circulating monocytes in part, we examined the shedding of microvesicles, that are known to carry mRNA as cargos, the cellular viability, and the mRNA expression for proopiomelanocortin (POMC) of human monocytic cells after RF application.  

Methods:

RF probe and the counter electrode were inserted into suspension of THP-1 cells in a microtube.  Continuous RF at 50°C or 70°C and pulsed RF (< 43°C) were applied to the cell suspension.  Cellular viability and the shedding of microvesicles were evaluated by using flow cytometry.  The expression of mRNA was measured by the real-time RT-PCR.

Results:

Continuous RF currents for 90 s caused significant necrotic cell death, while pulsed RF for up to 15 min failed to provoke apoptotic or necrotic cell death.  Continuous RF currents but not pulsed RF increased the number of microvesicle shed from THP-1 cells at 4 h after RF application.  The mRNA for POMC was increased in survived cells at 24 h after RF application of every modality.  

Conclusions:

Continuous RF even at the lowest temperature setting, but not pulsed RF, provoked necrotic cell death of human monocytic cells.  The gene expression for endorphine (mRNA for POMC) was increased by every RF application including prolonged pulsed RF. 

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